4ILG
Crystal structure of Aar2p in complex with the Prp8p RNaseH and Jab1/MPN domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.768, 63.739, 110.518 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 31.869 - 2.100 |
| R-factor | 0.1823 |
| Rwork | 0.180 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sbt 2og4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.207 | 1.154 |
| Number of reflections | 68679 | |
| <I/σ(I)> | 10.6 | 1.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1 M HEPES-NaOH, pH 7.5, 12 % (w/v) PEG 6000 and 0.6 M KCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
