4ILG

Crystal structure of Aar2p in complex with the Prp8p RNaseH and Jab1/MPN domains

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	A1 cistron-splicing factor AAR2	polymer	342	40219.9	1	UniProt (P32357) Pfam (PF20981) Pfam (PF05282)	Saccharomyces cerevisiae (Baker's yeast)
2	B (B)	Pre-mRNA-splicing factor 8	polymer	258	29501.1	1	UniProt (P33334) Pfam (PF12134)	Saccharomyces cerevisiae (Baker's yeast)
3	C (C)	Pre-mRNA-splicing factor 8	polymer	270	30490.3	1	UniProt (P33334) Pfam (PF08084)	Saccharomyces cerevisiae (Baker's yeast)
4	D (C)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
5	E, F, G (A, B, C)	water	water		18.0	689	Chemie (HOH)

Sequence modifications

A: 1 - 355 (UniProt: P32357)

PDB	External Database	Details
Ser 153	Leu 153	engineered mutation
Ser 154	Lys 154	engineered mutation
-	Leu 157	deletion
-	Gln 158	deletion
-	Lys 159	deletion
-	Ala 160	deletion
-	Gly 161	deletion
-	Lys 163	deletion
-	Met 164	deletion
-	Glu 165	deletion
-	Ala 166	deletion
-	Lys 167	deletion
-	Asn 168	deletion
-	Glu 169	deletion
-	Asp 170	deletion

B: 1836 - 2090 (UniProt: P33334)

PDB	External Database	Details
Gly 1833	-	expression tag
Ala 1834	-	expression tag
Met 1835	-	expression tag

C: 2147 - 2413 (UniProt: P33334)

PDB	External Database	Details
Gly 2144	-	expression tag
Ala 2145	-	expression tag
Met 2146	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	100211.3
Non-Polymers*	Number of molecules	1
	Total formula weight	238.3
All*	Total formula weight	100449.6

*Water molecules are not included.