4I3H
A three-gate structure of topoisomerase IV from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92000 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 160.600, 160.600, 280.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.823 - 3.700 |
| R-factor | 0.1886 |
| Rwork | 0.185 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rae 1ei1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.396 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.190 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.124 | 0.450 |
| Number of reflections | 39884 | |
| <I/σ(I)> | 3.1256 | 1.71 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.11 | 11.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 296 | 4% isopropanol, 50 mM sodium citrate, 600 nL + 400 nL (protein + precipitant) drop ratio, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
