4I3H

A three-gate structure of topoisomerase IV from Streptococcus pneumoniae

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA topoisomerase 4 subunit B,DNA topoisomerase 4 subunit A	polymer	1144	128323.9	2	UniProt (Q59961) UniProt (P72525) Pfam (PF02518) Pfam (PF00204) Pfam (PF01751) Pfam (PF00986) Pfam (PF00521)	Streptococcus pneumoniae TIGR4	Topoisomerase IV subunit A
2	C, E (G, E)	DNA (5'-D(*CP*AP*AP*AP*GP*GP*CP*GP*GP*TP*AP*AP*TP*AP*CP*GP*GP*TP*TP*AP*TP*CP*CP*AP*CP*AP*GP*AP*AP*TP*CP*AP*GP*G)-3')	polymer	34	10525.8	2		synthetic construct
3	D, F (H, F)	DNA (5'-D(*CP*CP*TP*GP*AP*TP*TP*CP*TP*GP*TP*GP*GP*AP*TP*AP*AP*CP*CP*GP*TP*AP*TP*TP*AP*CP*CP*GP*CP*CP*TP*TP*TP*G)-3')	polymer	34	10391.7	2		synthetic construct
4	G, H, I (A, B, H)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG) PubChem (888)
5	J (F)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

A, B: 1 - 999 (UniProt: Q59961)

PDB	External Database	Details
Asp 217	Asn 217	conflict
Ile 460	Val 460	conflict
Ala 996	Thr 644	conflict
His 1000	-	linker

A, B: 1001 - 1488 (UniProt: P72525)

PDB	External Database	Details
Thr 1257	Ile 257	conflict
Leu 1489	-	expression tag
Glu 1490	-	expression tag
His 1491	-	expression tag
His 1492	-	expression tag
His 1493	-	expression tag
His 1494	-	expression tag
His 1495	-	expression tag
His 1496	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	298482.7
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	298555.6

*Water molecules are not included.