4FBE
Crystal structure of the C136A/C164A variant of mitochondrial isoform of glutaminyl cyclase from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.928, 46.436, 74.150 |
| Unit cell angles | 84.93, 75.17, 74.47 |
Refinement procedure
| Resolution | 71.660 - 1.880 |
| R-factor | 0.17463 |
| Rwork | 0.171 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fai |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.392 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.663 | 1.990 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.075 | 0.342 |
| Number of reflections | 36763 | |
| <I/σ(I)> | 9.2 | 2.4 |
| Completeness [%] | 79.7 | 28.5 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 30% PEG4000, 0.2 M magnesium chloride, 1 mM PQ50, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
