4AYE
Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 E283AE304A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-05 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 187.413, 53.277, 130.084 |
Unit cell angles | 90.00, 117.74, 90.00 |
Refinement procedure
Resolution | 57.570 - 2.800 |
R-factor | 0.2078 |
Rwork | 0.207 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2w81 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.050 | 2.960 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.040 | 0.170 |
Number of reflections | 26592 | |
<I/σ(I)> | 16.7 | 4.3 |
Completeness [%] | 94.1 | 70.5 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 20% PEG 6000, 0.1M BICINE PH 9.0 |