4AYE

Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 E283AE304A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E (A, B, E)	COMPLEMENT FACTOR H	polymer	125	14410.3	3	UniProt (P08603) Pfam (PF00084)	HOMO SAPIENS (HUMAN)	H FACTOR 1
2	C, D, F (C, D, F)	FACTOR H BINDING PROTEIN	polymer	257	27583.9	3	UniProt (Q9JXV4) Pfam (PF20937) Pfam (PF08794)	NEISSERIA MENINGITIDIS MC58
3	G, H, I, J, K... (A, B, C, D, E...)	1,2-ETHANEDIOL	non-polymer		62.1	19	Chemie (EDO)
4	AA, BA, CA, DA, EA... (B, C, D, E, F...)	water	water		18.0	134	Chemie (HOH)

Sequence modifications

A, B, E: 321 - 443 (UniProt: P08603)

PDB	External Database	Details
Met 319	-	expression tag
Gly 320	-	expression tag
His 402	Tyr 402	variant

C, D, F: 73 - 320 (UniProt: Q9JXV4)

PDB	External Database	Details
Met 72	-	expression tag
Ala 283	Glu 283	engineered mutation
Ala 304	Glu 304	engineered mutation
Glu 321	-	expression tag
Leu 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	125982.4
Non-Polymers*	Number of molecules	19
	Total formula weight	1179.3
All*	Total formula weight	127161.7

*Water molecules are not included.