4ZCS
Crystal structure of the C-terminal catalytic domain of Plasmodium falciparum CTP:phosphocholine cytidylyltransferase in complex with CDP-choline
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-31 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979338 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 114.631, 149.132, 175.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 113.670 - 2.450 |
R-factor | 0.18325 |
Rwork | 0.181 |
R-free | 0.23413 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hl4 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.970 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 113.670 |
High resolution limit [Å] | 2.450 |
Number of reflections | 52521 |
<I/σ(I)> | 0.2 |
Completeness [%] | 99.6 |
Redundancy | 9.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.2M NaF, 20% PEG 3350 |