4OGR
crystal structure of P-TEFb complex with AFF4 and Tat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 184.908, 184.908, 360.399 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.015 - 3.000 |
| R-factor | 0.2056 |
| Rwork | 0.205 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4imy |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.805 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1419) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
| Rmerge | 0.222 | 0.044 | |
| Number of reflections | 73424 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 24.2 | 22.4 | 23.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291 | Crystallized from 25 mM HEPES pH 7.3, 0.2 M NaCl, 10 mM MgCl, 2.4 M Na formate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
