4AYE
Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 1 E283AE304A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-05 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.413, 53.277, 130.084 |
| Unit cell angles | 90.00, 117.74, 90.00 |
Refinement procedure
| Resolution | 57.570 - 2.800 |
| R-factor | 0.2078 |
| Rwork | 0.207 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w81 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.050 | 2.960 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.040 | 0.170 |
| Number of reflections | 26592 | |
| <I/σ(I)> | 16.7 | 4.3 |
| Completeness [%] | 94.1 | 70.5 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 20% PEG 6000, 0.1M BICINE PH 9.0 |
