3UV4

Crystal Structure of the second bromodomain of human Transcription initiation factor TFIID subunit 1 (TAF1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-06-04
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.52
Spacegroup name	P 21 21 21
Unit cell lengths	57.450, 57.800, 123.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.960 - 1.890
R-factor	0.1924
Rwork	0.191
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ensemble of PDB entries 3HMH 2grc 2oo1 2oss 2ouo 3dai 3d7c 3dwy 3g0l 3gg3
RMSD bond length	0.015
RMSD bond angle	1.356
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.960	28.900	1.990
High resolution limit [Å]	1.890	5.980	1.890
Rmerge	0.074	0.020	0.763
Total number of observations		5192	22387
Number of reflections	33417
<I/σ(I)>	14.4	29.2	1
Completeness [%]	99.2	99.1	97.8
Redundancy	4.7	4.3	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.056M NaH2PO4 1.344M K2HPO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K