3THT
Crystal structure and RNA binding properties of the RRM/AlkB domains in human ABH8, an enzyme catalyzing tRNA hypermodification, Northeast Structural Genomics Consortium Target HR5601B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.166, 73.279, 149.814 |
| Unit cell angles | 90.00, 112.73, 90.00 |
Refinement procedure
| Resolution | 39.060 - 3.010 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3thp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2 & Xtalview) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.082 | 0.304 |
| Number of reflections | 29876 | |
| <I/σ(I)> | 16.4 | 3.7 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 5.6 | 293 | 2.8 mM Mn2Cl and 8.6 mM 2OG and a precipitant containing 28.5-30.5% (w/v) PEG 4K, 15% (v/v) glycerol, 170 mM NH4(CH3COO), 85 mM Na-citrate, pH 5.6, microbatch under oil, temperature 293K |
