3THT

Crystal structure and RNA binding properties of the RRM/AlkB domains in human ABH8, an enzyme catalyzing tRNA hypermodification, Northeast Structural Genomics Consortium Target HR5601B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Alkylated DNA repair protein alkB homolog 8	polymer	345	39359.7	4	UniProt (Q96BT7) Pfam (PF09004) Pfam (PF00076) Pfam (PF13532)	Homo sapiens (human)	Probable alpha-ketoglutarate-dependent dioxygenase ABH8
2	E, H, K, N (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	F, I, L, O (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
4	G, J, M, P (A, B, C, D)	2-OXOGLUTARIC ACID	non-polymer		146.1	4	Chemie (AKG)
5	Q, R, S, T (A, B, C, D)	water	water		18.0	100	Chemie (HOH)

Sequence modifications

A, B, C, D: 25 - 355 (UniProt: Q96BT7)

PDB	External Database	Details
Asn 356	-	expression tag
Leu 357	-	expression tag
Tyr 358	-	expression tag
Phe 359	-	expression tag
Gln 360	-	expression tag
Gly 361	-	expression tag
Leu 362	-	expression tag
Glu 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	157438.7
Non-Polymers*	Number of molecules	12
	Total formula weight	1065.8
All*	Total formula weight	158504.5

*Water molecules are not included.