3QB1

Interleukin-2 mutant D10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	200
Detector technology	CCD
Collection date	2010-07-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	55.873, 75.019, 76.812
Unit cell angles	101.46, 102.29, 104.31

Refinement procedure

Resolution	42.400 - 3.100
R-factor	0.224
Rwork	0.221
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m47
RMSD bond length	0.009
RMSD bond angle	1.023
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_621)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.056	0.024	0.440
Number of reflections	20383
<I/σ(I)>	10.5
Completeness [%]	99.0	99.5	98.4
Redundancy	2.2	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	298	50 mM HEPES, 200 mM NaCl, 30% PEG-4000, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K