3OTF

Structural basis for the cAMP-dependent gating in human HCN4 channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-10-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97923
Spacegroup name	I 4 2 2
Unit cell lengths	69.264, 69.264, 191.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.23857
Rwork	0.237
R-free	0.27464
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q43
RMSD bond length	0.009
RMSD bond angle	1.082
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.053
Number of reflections	9567
<I/σ(I)>		94.2	5.2
Completeness [%]	94.0	85	95.9
Redundancy		20.2	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	12% ethanol, sodium citrate, 200 mM Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K