3OTF
Structural basis for the cAMP-dependent gating in human HCN4 channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97923 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 69.264, 69.264, 191.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.23857 |
| Rwork | 0.237 |
| R-free | 0.27464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q43 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.053 | ||
| Number of reflections | 9567 | ||
| <I/σ(I)> | 94.2 | 5.2 | |
| Completeness [%] | 94.0 | 85 | 95.9 |
| Redundancy | 20.2 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 12% ethanol, sodium citrate, 200 mM Li2SO4, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
