3I3J
Crystal Structure of the Bromodomain of Human EP300
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.899, 86.186, 149.897 |
Unit cell angles | 90.00, 96.98, 90.00 |
Refinement procedure
Resolution | 32.302 - 2.330 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dwy 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c |
RMSD bond length | 0.007 |
RMSD bond angle | 0.997 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.580 | 2.460 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.117 | 0.820 |
Number of reflections | 69648 | |
<I/σ(I)> | 10.3 | 2 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 4.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 30w/v PEG 3350, 0.2M ammonium_sulfate, 5.5pH Bis-Tris, VAPOR DIFFUSION, SITTING DROP, temperature 277K |