3HMH

Crystal structure of the second bromodomain of human TBP-associated factor RNA polymerase 1-like (TAF1L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-03-21
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5
Spacegroup name	P 32 2 1
Unit cell lengths	55.139, 55.139, 84.441
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.150 - 2.050
R-factor	0.185
Rwork	0.181
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c 3dwy
RMSD bond length	0.016
RMSD bond angle	1.385
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.150	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.075	0.687
Number of reflections	9764
<I/σ(I)>	15.6	2.7
Completeness [%]	100.0	100
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	15w/v PEG_3350, 0.17M ammonium_sulfate, 15w/v glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K