3HMF
Crystal Structure of the second Bromodomain of Human Poly-bromodomain containing protein 1 (PB1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-01-27 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.810, 49.360, 33.610 |
Unit cell angles | 90.00, 97.91, 90.00 |
Refinement procedure
Resolution | 27.177 - 1.630 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c 3dwy |
RMSD bond length | 0.005 |
RMSD bond angle | 0.848 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.150 | 1.720 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.041 | 0.274 |
Number of reflections | 17021 | |
<I/σ(I)> | 18.4 | 4.4 |
Completeness [%] | 97.6 | 92.8 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.01M Zn_Cl, 15w/v PEG_6000, 10v/v ethylene_glycol, 5.5pH MES, VAPOR DIFFUSION, SITTING DROP, temperature 277K |