3GIS
Crystal Structure of Na-free Thrombin in Complex with Thrombomodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.250, 100.340, 229.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.900 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.150 | 0.339 |
Number of reflections | 58334 | |
<I/σ(I)> | 8.9 | 3.5 |
Completeness [%] | 96.0 | 95 |
Redundancy | 4.7 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 294 | 0.2M LiSO4, 22% PEG3350, pH7.0, vapor diffusion, temperature 294K, VAPOR DIFFUSION |