3G0J
Crystal Structure of the fifth Bromodomain of Human Poly-bromodomain containing protein 1 (PB1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 36.570, 57.370, 137.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.450 - 1.780 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ensemble of 3DWY 3dai 3d7c 2ouo 2oss 2oo1 2nxb 2grc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.373 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.2) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.831 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.079 | 0.703 |
| Number of reflections | 28304 | |
| <I/σ(I)> | 18.2 | 2.6 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 8.8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2M Na_nitrate, 25% w/v PEG 3350, 5% v/v ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
