3E7B
Crystal Structure of Protein Phosphatase-1 Bound to the natural toxin inhibitor Tautomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-04-18 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.760, 78.519, 130.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.630 - 1.700 |
| R-factor | 0.15408 |
| Rwork | 0.153 |
| R-free | 0.17521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e7a |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.571 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 74099 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.2 | 93.3 |
| Redundancy | 5.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 298 | 0.1M Tris, 30% PEG 6K, 1M Lithium Sulfate, pH 8.0, under paraffin oil, temperature 298K |
