3I3J
Crystal Structure of the Bromodomain of Human EP300
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.899, 86.186, 149.897 |
| Unit cell angles | 90.00, 96.98, 90.00 |
Refinement procedure
| Resolution | 32.302 - 2.330 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dwy 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.997 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.580 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.117 | 0.820 |
| Number of reflections | 69648 | |
| <I/σ(I)> | 10.3 | 2 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 30w/v PEG 3350, 0.2M ammonium_sulfate, 5.5pH Bis-Tris, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
