3HMF
Crystal Structure of the second Bromodomain of Human Poly-bromodomain containing protein 1 (PB1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.810, 49.360, 33.610 |
| Unit cell angles | 90.00, 97.91, 90.00 |
Refinement procedure
| Resolution | 27.177 - 1.630 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nxb 2oo1 2oss 2ouo 2rfj 3dai 3d7c 3dwy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.848 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.150 | 1.720 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.041 | 0.274 |
| Number of reflections | 17021 | |
| <I/σ(I)> | 18.4 | 4.4 |
| Completeness [%] | 97.6 | 92.8 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.01M Zn_Cl, 15w/v PEG_6000, 10v/v ethylene_glycol, 5.5pH MES, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
