2VD6
Human adenylosuccinate lyase in complex with its substrate N6-(1,2- Dicarboxyethyl)-AMP, and its products AMP and fumarate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-23 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.300, 128.100, 190.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.194 |
Rwork | 0.192 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j91 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.088 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0040) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.200 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.380 |
Number of reflections | 144136 | |
<I/σ(I)> | 21.3 | 5.5 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 7.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |