2VD6
Human adenylosuccinate lyase in complex with its substrate N6-(1,2- Dicarboxyethyl)-AMP, and its products AMP and fumarate.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001666 | biological_process | response to hypoxia |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0004018 | molecular_function | N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006164 | biological_process | purine nucleotide biosynthetic process |
| A | 0006167 | biological_process | AMP biosynthetic process |
| A | 0006177 | biological_process | GMP biosynthetic process |
| A | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| A | 0007584 | biological_process | response to nutrient |
| A | 0009060 | biological_process | aerobic respiration |
| A | 0009152 | biological_process | purine ribonucleotide biosynthetic process |
| A | 0009168 | biological_process | purine ribonucleoside monophosphate biosynthetic process |
| A | 0014850 | biological_process | response to muscle activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0032991 | cellular_component | protein-containing complex |
| A | 0042594 | biological_process | response to starvation |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0044208 | biological_process | 'de novo' AMP biosynthetic process |
| A | 0044209 | biological_process | AMP salvage |
| A | 0070626 | molecular_function | (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity |
| A | 0097294 | biological_process | 'de novo' XMP biosynthetic process |
| B | 0001666 | biological_process | response to hypoxia |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0004018 | molecular_function | N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006164 | biological_process | purine nucleotide biosynthetic process |
| B | 0006167 | biological_process | AMP biosynthetic process |
| B | 0006177 | biological_process | GMP biosynthetic process |
| B | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| B | 0007584 | biological_process | response to nutrient |
| B | 0009060 | biological_process | aerobic respiration |
| B | 0009152 | biological_process | purine ribonucleotide biosynthetic process |
| B | 0009168 | biological_process | purine ribonucleoside monophosphate biosynthetic process |
| B | 0014850 | biological_process | response to muscle activity |
| B | 0016829 | molecular_function | lyase activity |
| B | 0032991 | cellular_component | protein-containing complex |
| B | 0042594 | biological_process | response to starvation |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0044208 | biological_process | 'de novo' AMP biosynthetic process |
| B | 0044209 | biological_process | AMP salvage |
| B | 0070626 | molecular_function | (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity |
| B | 0097294 | biological_process | 'de novo' XMP biosynthetic process |
| C | 0001666 | biological_process | response to hypoxia |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0004018 | molecular_function | N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006164 | biological_process | purine nucleotide biosynthetic process |
| C | 0006167 | biological_process | AMP biosynthetic process |
| C | 0006177 | biological_process | GMP biosynthetic process |
| C | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| C | 0007584 | biological_process | response to nutrient |
| C | 0009060 | biological_process | aerobic respiration |
| C | 0009152 | biological_process | purine ribonucleotide biosynthetic process |
| C | 0009168 | biological_process | purine ribonucleoside monophosphate biosynthetic process |
| C | 0014850 | biological_process | response to muscle activity |
| C | 0016829 | molecular_function | lyase activity |
| C | 0032991 | cellular_component | protein-containing complex |
| C | 0042594 | biological_process | response to starvation |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0044208 | biological_process | 'de novo' AMP biosynthetic process |
| C | 0044209 | biological_process | AMP salvage |
| C | 0070626 | molecular_function | (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity |
| C | 0097294 | biological_process | 'de novo' XMP biosynthetic process |
| D | 0001666 | biological_process | response to hypoxia |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0004018 | molecular_function | N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0006164 | biological_process | purine nucleotide biosynthetic process |
| D | 0006167 | biological_process | AMP biosynthetic process |
| D | 0006177 | biological_process | GMP biosynthetic process |
| D | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| D | 0007584 | biological_process | response to nutrient |
| D | 0009060 | biological_process | aerobic respiration |
| D | 0009152 | biological_process | purine ribonucleotide biosynthetic process |
| D | 0009168 | biological_process | purine ribonucleoside monophosphate biosynthetic process |
| D | 0014850 | biological_process | response to muscle activity |
| D | 0016829 | molecular_function | lyase activity |
| D | 0032991 | cellular_component | protein-containing complex |
| D | 0042594 | biological_process | response to starvation |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0044208 | biological_process | 'de novo' AMP biosynthetic process |
| D | 0044209 | biological_process | AMP salvage |
| D | 0070626 | molecular_function | (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity |
| D | 0097294 | biological_process | 'de novo' XMP biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 21 |
| Details | BINDING SITE FOR RESIDUE AMP A 1000 |
| Chain | Residue |
| A | ARG85 |
| A | ARG338 |
| A | FUM1001 |
| A | CL2001 |
| A | HOH2054 |
| A | HOH2251 |
| A | HOH2252 |
| A | HOH2253 |
| B | HIS159 |
| D | ARG20 |
| D | TYR21 |
| A | HIS86 |
| D | MET299 |
| D | ARG303 |
| A | ASP87 |
| A | SER112 |
| A | GLN241 |
| A | ARG329 |
| A | LEU331 |
| A | SER334 |
| A | ALA335 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE FUM A 1001 |
| Chain | Residue |
| A | HIS86 |
| A | MET89 |
| A | THR111 |
| A | SER112 |
| A | GLN241 |
| A | AMP1000 |
| A | HOH2255 |
| A | HOH2256 |
| B | THR158 |
| B | HIS159 |
| D | LYS295 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL A 1003 |
| Chain | Residue |
| A | LYS35 |
| A | TRP39 |
| A | ASP87 |
| A | GLY116 |
| A | ASP120 |
| A | SER334 |
| A | ARG338 |
| A | HOH2161 |
| A | HOH2257 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL A 2001 |
| Chain | Residue |
| A | AMP1000 |
| D | ASN297 |
| D | MET299 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CL A 2002 |
| Chain | Residue |
| A | THR111 |
| A | TYR114 |
| A | ARG196 |
| A | LYS199 |
| A | GLY200 |
| site_id | AC6 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE 2SA B 1002 |
| Chain | Residue |
| A | THR158 |
| A | HIS159 |
| B | ARG85 |
| B | HIS86 |
| B | ASP87 |
| B | THR111 |
| B | SER112 |
| B | GLN241 |
| B | ARG329 |
| B | SER334 |
| B | ALA335 |
| B | ARG338 |
| B | CL2001 |
| B | HOH2038 |
| B | HOH2051 |
| B | HOH2130 |
| B | HOH2161 |
| B | HOH2163 |
| B | HOH2165 |
| B | HOH2166 |
| C | ARG20 |
| C | TYR21 |
| C | LYS295 |
| C | MET299 |
| C | ARG303 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE GOL B 1003 |
| Chain | Residue |
| B | LYS35 |
| B | ASP87 |
| B | GLY116 |
| B | ASP120 |
| B | SER334 |
| B | ARG338 |
| B | HOH2168 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE GOL B 1004 |
| Chain | Residue |
| B | ASP120 |
| B | ARG337 |
| B | ARG338 |
| B | LEU341 |
| B | ALA342 |
| B | HOH2131 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE CL B 2001 |
| Chain | Residue |
| B | 2SA1002 |
| B | HOH2166 |
| C | ASN297 |
| site_id | BC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CL B 2002 |
| Chain | Residue |
| B | THR111 |
| B | TYR114 |
| B | ARG196 |
| B | LYS199 |
| B | GLY200 |
| site_id | BC2 |
| Number of Residues | 19 |
| Details | BINDING SITE FOR RESIDUE AMP C 1000 |
| Chain | Residue |
| C | ARG85 |
| C | HIS86 |
| C | ASP87 |
| C | SER112 |
| C | GLN241 |
| C | ARG329 |
| C | LEU331 |
| C | SER334 |
| C | ALA335 |
| C | ARG338 |
| C | FUM1001 |
| C | HOH2241 |
| C | HOH2242 |
| C | HOH2243 |
| D | HIS159 |
| B | ARG20 |
| B | TYR21 |
| B | MET299 |
| B | ARG303 |
| site_id | BC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE FUM C 1001 |
| Chain | Residue |
| B | LYS295 |
| B | ASN297 |
| C | HIS86 |
| C | THR111 |
| C | SER112 |
| C | GLN241 |
| C | AMP1000 |
| D | THR158 |
| D | HIS159 |
| site_id | BC4 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL C 1003 |
| Chain | Residue |
| C | LYS35 |
| C | TRP39 |
| C | ASP87 |
| C | GLY116 |
| C | ASP120 |
| C | SER334 |
| C | ARG338 |
| C | HOH2167 |
| C | HOH2245 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL C 2002 |
| Chain | Residue |
| C | TYR114 |
| C | ARG196 |
| C | LYS199 |
| C | GLY200 |
| site_id | BC6 |
| Number of Residues | 25 |
| Details | BINDING SITE FOR RESIDUE 2SA D 1002 |
| Chain | Residue |
| A | ARG20 |
| A | TYR21 |
| A | LYS295 |
| A | MET299 |
| A | ARG303 |
| C | THR158 |
| C | HIS159 |
| D | ARG85 |
| D | HIS86 |
| D | ASP87 |
| D | THR111 |
| D | SER112 |
| D | GLN241 |
| D | ARG329 |
| D | SER334 |
| D | ALA335 |
| D | ARG338 |
| D | CL2001 |
| D | HOH2067 |
| D | HOH2087 |
| D | HOH2244 |
| D | HOH2245 |
| D | HOH2246 |
| D | HOH2247 |
| D | HOH2249 |
| site_id | BC7 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE GOL D 1003 |
| Chain | Residue |
| D | LYS35 |
| D | TRP39 |
| D | ASP87 |
| D | GLY116 |
| D | ASP120 |
| D | SER334 |
| D | ARG338 |
| D | HOH2212 |
| D | HOH2213 |
| D | HOH2250 |
| site_id | BC8 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL D 2001 |
| Chain | Residue |
| A | ASN297 |
| A | MET299 |
| D | 2SA1002 |
| D | HOH2026 |
| site_id | BC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CL D 2002 |
| Chain | Residue |
| D | THR111 |
| D | TYR114 |
| D | ARG196 |
| D | LYS199 |
| D | GLY200 |
Functional Information from PROSITE/UniProt
| site_id | PS00163 |
| Number of Residues | 10 |
| Details | FUMARATE_LYASES Fumarate lyases signature. GSsaMpYKrN |
| Chain | Residue | Details |
| A | GLY288-ASN297 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000269|PubMed:22812634 |
| Chain | Residue | Details |
| A | HIS159 | |
| A | SER289 | |
| B | HIS159 | |
| B | SER289 | |
| C | HIS159 | |
| C | SER289 | |
| D | HIS159 | |
| D | SER289 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | ARG20 | |
| A | ARG303 | |
| B | ARG20 | |
| B | ARG303 | |
| C | ARG20 | |
| C | ARG303 | |
| D | ARG20 | |
| D | ARG303 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 24 |
| Details | BINDING: in other chain |
| Chain | Residue | Details |
| A | ARG85 | |
| B | ARG329 | |
| B | SER334 | |
| B | ARG338 | |
| C | ARG85 | |
| C | THR111 | |
| C | GLN241 | |
| C | ARG329 | |
| C | SER334 | |
| C | ARG338 | |
| D | ARG85 | |
| A | THR111 | |
| D | THR111 | |
| D | GLN241 | |
| D | ARG329 | |
| D | SER334 | |
| D | ARG338 | |
| A | GLN241 | |
| A | ARG329 | |
| A | SER334 | |
| A | ARG338 | |
| B | ARG85 | |
| B | THR111 | |
| B | GLN241 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: N-acetylalanine => ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | ALA2 | |
| B | ALA2 | |
| C | ALA2 | |
| D | ALA2 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS147 | |
| A | LYS295 | |
| B | LYS147 | |
| B | LYS295 | |
| C | LYS147 | |
| C | LYS295 | |
| D | LYS147 | |
| D | LYS295 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) => ECO:0007744|PubMed:25114211 |
| Chain | Residue | Details |
| A | LYS415 | |
| B | LYS415 | |
| C | LYS415 | |
| D | LYS415 |
