Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.110, 54.250, 61.040 |
Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
Resolution | 37.420 - 1.930 |
R-factor | 0.214 |
Rwork | 0.210 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qlp |
RMSD bond length | 0.013 |
RMSD bond angle | 1.478 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.060 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.070 | 0.650 |
Number of reflections | 29097 | |
<I/σ(I)> | 15.3 | 3 |
Completeness [%] | 96.9 | 96 |
Redundancy | 4.7 | 4.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 1 MICROLITER OF 15 MG/ML RECOMBINANT RAT CBG AND 1 MICROLITER OF RESERVOIR SOLUTION (30% (W/V) PEG 4000, 300 MM LI2SO4, 100 MM TRIS-HCL, PH 8.5) AGAINST 700 MICROLITER OF RESERVOIR SOLUTION |