Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.110, 54.250, 61.040 |
| Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
| Resolution | 37.420 - 1.930 |
| R-factor | 0.214 |
| Rwork | 0.210 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qlp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.478 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.060 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.070 | 0.650 |
| Number of reflections | 29097 | |
| <I/σ(I)> | 15.3 | 3 |
| Completeness [%] | 96.9 | 96 |
| Redundancy | 4.7 | 4.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 1 MICROLITER OF 15 MG/ML RECOMBINANT RAT CBG AND 1 MICROLITER OF RESERVOIR SOLUTION (30% (W/V) PEG 4000, 300 MM LI2SO4, 100 MM TRIS-HCL, PH 8.5) AGAINST 700 MICROLITER OF RESERVOIR SOLUTION |
