1XDD
X-ray structure of LFA-1 I-domain in complex with LFA703 at 2.2A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.80074 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 116.700, 116.700, 82.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.16368 |
| Rwork | 0.161 |
| R-free | 0.20643 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cqp |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.512 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 28795 | |
| <I/σ(I)> | 22.7 | 7.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | ammonium acetate, sodium acetate, glycerol, PEG4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
