1VSO

Crystal Structure of the Ligand-Binding Core of iGluR5 in Complex With the Antagonist (S)-ATPO at 1.85 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-13
Detector	MAR CCD 165 mm
Spacegroup name	P 64
Unit cell lengths	108.900, 108.900, 51.092
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.660 - 1.850
R-factor	0.202
Rwork	0.202
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n0t CHAIN A
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	29461
<I/σ(I)>	23.1	3.8
Completeness [%]	99.7	99.9
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	279	20% PEG 4000, 0.3 M lithium sulfate, 0.1 M cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 6.50