1UKL

Crystal structure of Importin-beta and SREBP-2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	CCD
Collection date	2002-11-24
Detector	Bruker DIP-6040
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	101.092, 113.285, 240.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 3.000
Rwork	0.239
R-free	0.29600 *
Structure solution method	SAD
Starting model (for MR)	1qgk 1am9
RMSD bond length	0.008 *
RMSD bond angle	1.427 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	3.100
High resolution limit [Å]	3.000	3.000
Rmerge	0.064	0.300
Number of reflections	55607 *	5720 *
<I/σ(I)>	27.7	3
Completeness [%]	99.7	97.5
Redundancy	5.9	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	288	PEG 8000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	6.6	288	PEG 8000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	50 (mM)	pH6.6
2	1	reservoir	PEG8000	5-6 (%)
3	1	reservoir		30 (mM)
4	1	reservoir	glycerol	20 (%)
5	1	drop	MES	50 (mM)	pH6.6
6	1	drop	PEG8000	5-6 (%)
7	1	drop		30 (mM)
8	1	drop	glycerol	10 (%)