1OC0
plasminogen activator inhibitor-1 complex with somatomedin B domain of vitronectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-15 |
| Detector | APS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.501, 90.087, 100.163 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.280 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b3k |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BEAST |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.370 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.112 | 0.733 |
| Number of reflections | 20251 | 2204 * |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 100.0 | 99.95 * |
| Redundancy | 12.2 | 7.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 4 * | 10MM NAOAC, 50MM NACL, 2-9% PEG12000,25% PEG400, 50MM HEPES, PH 7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 10 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | PEG12000 | 2-9 (%(w/v)) | |
| 5 | 1 | reservoir | HEPES | 50 (mM) | pH7.4 |
