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- PDB-8fab: CRYSTAL STRUCTURE OF THE FAB FRAGMENT FROM THE HUMAN MYELOMA IMMU... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8fab | ||||||
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Title | CRYSTAL STRUCTURE OF THE FAB FRAGMENT FROM THE HUMAN MYELOMA IMMUNOGLOBULIN IGG HIL AT 1.8 ANGSTROMS RESOLUTION | ||||||
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![]() | IMMUNOGLOBULIN | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : / : ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Saul, F.A. / Poljak, R.J. | ||||||
![]() | ![]() Title: Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 1. X-ray crystallography, site-directed mutagenesis, and modeling of the complex with hapten. Authors: Strong, R.K. / Campbell, R. / Rose, D.R. / Petsko, G.A. / Sharon, J. / Margolies, M.N. #1: ![]() Title: Amino Acid Sequence of the VH Region of Human Myeloma Cryoimmunoglobulin Igg Hil Authors: Chiu, Y.-Y.H. / Lopez De Castro, J.A. / Poljak, R.J. #2: ![]() Title: Amino Acid Sequence of the Variable Region of the Light (Lambda) Chain from Human Myeloma Cryoimmunoglobulin Igg Hil Authors: Lopez De Castro, J.A. / Chiu, Y.-Y.H. / Poljak, R.J. #3: ![]() Title: Crystals of Fragment Fab': Preparation from Pepsin Digests of Human Igg Myeloma Proteins Authors: Rossi, G. / Choi, T.K. / Nisonoff, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 186.2 KB | Display | ![]() |
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PDB format | ![]() | 147.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 397.3 KB | Display | ![]() |
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Full document | ![]() | 410.7 KB | Display | |
Data in XML | ![]() | 17.8 KB | Display | |
Data in CIF | ![]() | 31.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Atom site foot note | 1: RESIDUE 141 IN CHAINS *A* AND *C* ARE CIS PROLINES. 2: RESIDUES 155 AND 157 IN CHAINS *B* AND *D* ARE CIS PROLINES. |
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Components
#1: Antibody | Mass: 22767.197 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Antibody | Mass: 24090.219 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #3: Water | ChemComp-HOH / | Sequence details | IN THE SEQUENCE DATA BASE, THE SEQUENCE FOR THE HEAVY CHAIN IS INCOMPLETE AND THE SEQUENCE FOR THE ...IN THE SEQUENCE DATA BASE, THE SEQUENCE FOR THE HEAVY CHAIN IS INCOMPLETE | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.77 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusionDetails: referred to 'Rose,D.R.', (1990) Proc.Natl.Acad.Sci.U.S.A., 87, 338-342 | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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Processing
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Refinement | Resolution: 1.8→6 Å / σ(F): 2.5 /
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Refinement step | Cycle: LAST / Resolution: 1.8→6 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 6 Å / Num. reflection obs: 64477 / σ(F): 2.5 / Rfactor obs: 0.173 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2.96 |