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Open data
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Basic information
Entry | Database: PDB / ID: 3ejz | ||||||
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Title | Structure of E203V mutant E.coli Cl-/H+ exchanger, CLC-ec1 | ||||||
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![]() | IMMUNE SYSTEM/PROTON TRANSPORT / membrane protein / Cl-/H+ exchanger / Antiport / Cell inner membrane / Cell membrane / Chloride / Ion transport / Stress response / Transmembrane / Transport / IMMUNE SYSTEM-PROTON TRANSPORT COMPLEX | ||||||
Function / homology | ![]() chloride:proton antiporter activity / cellular stress response to acidic pH / voltage-gated chloride channel activity / chloride transmembrane transport / proton transmembrane transport / B cell differentiation / extracellular region / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lim, H.-H. / Miller, C. | ||||||
![]() | ![]() Title: Intracellular proton-transfer mutants in a CLC Cl-/H+ exchanger. Authors: Lim, H.H. / Miller, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 332.6 KB | Display | ![]() |
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PDB format | ![]() | 270 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 486.8 KB | Display | ![]() |
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Full document | ![]() | 573.5 KB | Display | |
Data in XML | ![]() | 67.6 KB | Display | |
Data in CIF | ![]() | 90.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3ejyC ![]() 1otsS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
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Components
#1: Protein | Mass: 50360.418 Da / Num. of mol.: 2 / Mutation: E203V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 23693.918 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Antibody | Mass: 23088.443 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #4: Chemical | ChemComp-BR / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.64 Å3/Da / Density % sol: 66.21 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 36% (w/v) PEG 300, 20 mM NaBr, 50 mM tris-SO4, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jan 8, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.919 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→60 Å / Num. all: 61705 / Num. obs: 58605 / % possible obs: 99.9 % / Observed criterion σ(I): 1 / Redundancy: 7 % / Biso Wilson estimate: 88.7 Å2 / Rmerge(I) obs: 0.072 / Rsym value: 0.072 / Net I/σ(I): 18.9 |
Reflection shell | Resolution: 2.9→3.06 Å / Redundancy: 7 % / Rmerge(I) obs: 0.54 / Mean I/σ(I) obs: 2.6 / Num. unique all: 9005 / Rsym value: 0.54 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1ots as a starting model Resolution: 2.9→58.76 Å / Cor.coef. Fo:Fc: 0.915 / Cor.coef. Fo:Fc free: 0.908 / Cross valid method: THROUGHOUT / σ(F): 2 / ESU R: 1.133 / ESU R Free: 0.394 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 97.464 Å2
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Refinement step | Cycle: LAST / Resolution: 2.9→58.76 Å
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Refine LS restraints |
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Refine LS restraints NCS | Dom-ID: 1 / Auth asym-ID: A / Ens-ID: 1 / Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 2.9→2.975 Å / Total num. of bins used: 20
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