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Open data
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Basic information
Entry | Database: PDB / ID: 2fee | ||||||
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Title | Structure of the Cl-/H+ exchanger CLC-ec1 from E.Coli in NaBr | ||||||
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![]() | PROTON TRANSPORT / MEMBRANE PROTEIN / CLC-ec1 / CLCA_ECOLI / Chloride/Proton exchange transporter | ||||||
Function / homology | ![]() chloride:proton antiporter activity / cellular stress response to acidic pH / voltage-gated chloride channel activity / chloride transmembrane transport / proton transmembrane transport / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Accardi, A. / Walden, M.P. / Nguitragool, W. / Jayaram, H. / Williams, C. / Miller, C. | ||||||
![]() | ![]() Title: Separate ion pathways in a Cl-/H+ exchanger Authors: Accardi, A. / Walden, M.P. / Nguitragool, W. / Jayaram, H. / Williams, C. / Miller, C. #1: ![]() Title: Gating the selectivity filter in ClC chloride channels Authors: Dutzler, R. / Cambell, E.B. / Mackinnon, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 329.3 KB | Display | ![]() |
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PDB format | ![]() | 268.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 480.3 KB | Display | ![]() |
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Full document | ![]() | 543.6 KB | Display | |
Data in XML | ![]() | 64.2 KB | Display | |
Data in CIF | ![]() | 87.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2fecC ![]() 2fedC ![]() 1otsS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: ARG / Beg label comp-ID: ARG / End auth comp-ID: ALA / End label comp-ID: ALA / Refine code: 2 / Auth seq-ID: 18 - 458 / Label seq-ID: 18 - 458
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Components
#1: Protein | Mass: 49658.695 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 23823.031 Da / Num. of mol.: 2 / Fragment: Heavy Chain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Antibody | Mass: 23088.443 Da / Num. of mol.: 2 / Fragment: Light Chain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.73 Å3/Da / Density % sol: 67.05 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 100 mM NaBr, PEG 400 37% , 0.05 M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9198 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→128.04 Å / Num. obs: 43955 / % possible obs: 99 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.071 / Rsym value: 0.071 / Net I/σ(I): 19.1 |
Reflection shell | Resolution: 3.2→3.3 Å / Rmerge(I) obs: 0.523 / Mean I/σ(I) obs: 2.3 / Rsym value: 0.523 / % possible all: 98.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1OTS Resolution: 3.2→128.04 Å / Cor.coef. Fo:Fc: 0.925 / Cor.coef. Fo:Fc free: 0.902 / SU B: 29.189 / SU ML: 0.475 / Cross valid method: THROUGHOUT / σ(F): 3 / ESU R: 1.45 / ESU R Free: 0.526 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 119.602 Å2
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Refinement step | Cycle: LAST / Resolution: 3.2→128.04 Å
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Refine LS restraints |
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Refine LS restraints NCS | Dom-ID: 1 / Auth asym-ID: A / Ens-ID: 1 / Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 3.2→3.283 Å / Total num. of bins used: 20
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