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- PDB-6k5a: Crystal structure of the E148D/R147A/F317A mutant in presence of ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6k5a | |||||||||
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Title | Crystal structure of the E148D/R147A/F317A mutant in presence of 200 mM NaBr | |||||||||
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![]() | MEMBRANE PROTEIN / Cl- / H+ antiporter / CLC transporter | |||||||||
Function / homology | ![]() voltage-gated chloride channel activity / antiporter activity / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Park, K. / Lim, H.H. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Mutation of external glutamate residue reveals a new intermediate transport state and anion binding site in a CLC Cl-/H+antiporter. Authors: Park, K. / Lee, B.C. / Lim, H.H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 333.4 KB | Display | ![]() |
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PDB format | ![]() | 269.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6ad7C ![]() 6ad8C ![]() 6adaC ![]() 6adbC ![]() 6adcC ![]() 6k5dC ![]() 6k5fC ![]() 6k5iC ![]() 4eneS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50214.168 Da / Num. of mol.: 2 / Mutation: R147A, E148D, F317A Source method: isolated from a genetically manipulated source Details: SF file contains Friedel pairs. / Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 23823.031 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: SF file contains Friedel pairs. / Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Antibody | Mass: 23088.443 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: SF file contains Friedel pairs. / Source: (gene. exp.) ![]() ![]() ![]() ![]() #4: Chemical | ChemComp-BR / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.87 Å3/Da / Density % sol: 68.19 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 9 / Details: PEG 400 25% (V/V), 200 mM NaBr 100 mM Glycine |
-Data collection
Diffraction | Mean temperature: 77.36 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Apr 8, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 3.16→39.7 Å / Num. obs: 98471 / % possible obs: 99.9 % / Redundancy: 3.5 % / CC1/2: 0.98 / Rmerge(I) obs: 0.081 / Net I/σ(I): 20.2 |
Reflection shell | Resolution: 3.16→3.21 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.615 / Mean I/σ(I) obs: 1.56 / Num. unique obs: 4868 / CC1/2: 0.795 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4ENE Resolution: 3.162→39.686 Å / SU ML: 0.44 / Cross valid method: FREE R-VALUE / σ(F): 1.97 / Phase error: 31.36
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.162→39.686 Å
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Refine LS restraints |
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LS refinement shell |
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