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- PDB-6k5i: Crystal structure of the E148D/R147A/F317A mutant CLC-ec1 in the ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6k5i | |||||||||
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Title | Crystal structure of the E148D/R147A/F317A mutant CLC-ec1 in the presence of 20 mM NaBr | |||||||||
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![]() | MEMBRANE PROTEIN / Cl-/H+ antiporter / CLC transporter | |||||||||
Function / homology | ![]() voltage-gated chloride channel activity / antiporter activity / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Park, K. / Lim, H.H. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Mutation of external glutamate residue reveals a new intermediate transport state and anion binding site in a CLC Cl-/H+antiporter. Authors: Park, K. / Lee, B.C. / Lim, H.H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 333.9 KB | Display | ![]() |
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PDB format | ![]() | 269.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 59.6 KB | Display | |
Data in CIF | ![]() | 82 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ad7C ![]() 6ad8C ![]() 6adaC ![]() 6adbC ![]() 6adcC ![]() 6k5aC ![]() 6k5dC ![]() 6k5fC ![]() 4eneS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50214.168 Da / Num. of mol.: 2 / Mutation: R147A, E148D, F317A Source method: isolated from a genetically manipulated source Details: SF file contains Friedel pairs. / Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 23823.031 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: SF file contains Friedel pairs. / Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Antibody | Mass: 23088.443 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: SF file contains Friedel pairs. / Source: (gene. exp.) ![]() ![]() ![]() ![]() #4: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.74 Å3/Da / Density % sol: 67.11 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 400 23% (v/v), 20 mM Na/K tartrate, 100 mM Tris-SO4 |
-Data collection
Diffraction | Mean temperature: 77.36 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Apr 2, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 3.02→42.9 Å / Num. obs: 109854 / % possible obs: 99.9 % / Redundancy: 3.5 % / CC1/2: 0.96 / Rmerge(I) obs: 0.074 / Net I/σ(I): 20.2 |
Reflection shell | Resolution: 3.02→3.07 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.569 / Mean I/σ(I) obs: 1.69 / Num. unique obs: 5476 / CC1/2: 0.813 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4ENE Resolution: 3.022→42.886 Å / SU ML: 0.44 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 28.81
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.022→42.886 Å
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Refine LS restraints |
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LS refinement shell |
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