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- PDB-2exy: Crystal structure of the E148Q Mutant of EcClC, Fab complexed in ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2exy | ||||||
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Title | Crystal structure of the E148Q Mutant of EcClC, Fab complexed in absence of bound ions | ||||||
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![]() | MEMBRANE PROTEIN / ClC family of channels and transporters / H+/Cl- antiporter / membrane protein-Fab complex | ||||||
Function / homology | ![]() chloride:proton antiporter activity / cellular stress response to acidic pH / voltage-gated chloride channel activity / immunoglobulin complex / immunoglobulin mediated immune response / chloride transmembrane transport / proton transmembrane transport / antigen binding / blood microparticle / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lobet, S. / Dutzler, R. | ||||||
![]() | ![]() Title: Ion-binding properties of the ClC chloride selectivity filter. Authors: Lobet, S. / Dutzler, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 331.2 KB | Display | ![]() |
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PDB format | ![]() | 268.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 485.3 KB | Display | ![]() |
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Full document | ![]() | 595.2 KB | Display | |
Data in XML | ![]() | 72.6 KB | Display | |
Data in CIF | ![]() | 98.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2exwC ![]() 2ez0C ![]() 1otuS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50389.418 Da / Num. of mol.: 2 / Mutation: E148Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 23823.031 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Antibody | Mass: 23088.443 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.02 Å3/Da / Density % sol: 69.43 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: peg 200/300 1:2, 150mM Tart, 50mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 23, 2004 |
Radiation | Monochromator: LN2 cooled fixed-exit Si(111) monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.919998 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. all: 59808 / Num. obs: 58313 / % possible obs: 97.5 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Biso Wilson estimate: 89.2 Å2 |
Reflection shell | Resolution: 3.05→3.16 Å / % possible all: 93.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 1OTU Resolution: 3.1→19.97 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 2651849.57 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 10 Å2 / ksol: 0.244759 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 77.8 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.1→19.97 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 3.1→3.29 Å / Rfactor Rfree error: 0.025 / Total num. of bins used: 6
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Xplor file | Serial no: 1 / Param file: protein_rep.param / Topol file: protein.top |