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- PDB-6app: Anti-Marburgvirus Nucleoprotein Single Domain Antibody A Complexe... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6app | ||||||
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Title | Anti-Marburgvirus Nucleoprotein Single Domain Antibody A Complexed with Nucleoprotein C-terminal domain | ||||||
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![]() | IMMUNE SYSTEM | ||||||
Function / homology | ![]() viral RNA genome packaging / helical viral capsid / viral budding via host ESCRT complex / viral nucleocapsid / host cell cytoplasm / ribonucleoprotein complex / RNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Taylor, A.B. / Garza, J.A. | ||||||
![]() | ![]() Title: Unveiling a Drift Resistant Cryptotope withinMarburgvirusNucleoprotein Recognized by Llama Single-Domain Antibodies. Authors: Garza, J.A. / Taylor, A.B. / Sherwood, L.J. / Hart, P.J. / Hayhurst, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 60 KB | Display | ![]() |
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PDB format | ![]() | 40.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.7 KB | Display | ![]() |
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Full document | ![]() | 421.5 KB | Display | |
Data in XML | ![]() | 12.3 KB | Display | |
Data in CIF | ![]() | 18.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4w2oC ![]() 4w2pC ![]() 4w2qC ![]() 6apoSC ![]() 6apqC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 14215.889 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: Semi-synthetic single pot library Nomad 1 based upon Lama glama Plasmid: PECAN73 / Production host: ![]() ![]() |
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#2: Protein | Mass: 12238.336 Da / Num. of mol.: 1 / Fragment: C-terminal domain residues 601-695 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: Musoke-80 / Gene: NP / Plasmid: pE-NP600 / Production host: ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.86 Å3/Da / Density % sol: 33.99 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / Details: 15% polyethylene glycol 6000, 5% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS HTC / Detector: IMAGE PLATE / Date: Mar 1, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→46.15 Å / Num. obs: 20487 / % possible obs: 99.2 % / Redundancy: 6.9 % / Biso Wilson estimate: 19 Å2 / Rpim(I) all: 0.039 / Rsym value: 0.089 / Net I/σ(I): 14.1 |
Reflection shell | Resolution: 1.75→1.84 Å / Redundancy: 6.9 % / Mean I/σ(I) obs: 2.6 / Num. unique obs: 2902 / Rpim(I) all: 0.281 / Rsym value: 0.64 / % possible all: 98.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6APO Resolution: 1.75→34.343 Å / SU ML: 0.22 / Cross valid method: THROUGHOUT / σ(F): 1.4 / Phase error: 20.68
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.1 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.75→34.343 Å
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Refine LS restraints |
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LS refinement shell |
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