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- PDB-4w2o: Anti-Marburgvirus Nucleoprotein Single Domain Antibody B Complexe... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4w2o | ||||||
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Title | Anti-Marburgvirus Nucleoprotein Single Domain Antibody B Complexed with Nucleoprotein C-terminal domain | ||||||
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![]() | IMMUNE SYSTEM | ||||||
Function / homology | ![]() viral RNA genome packaging / helical viral capsid / viral budding via host ESCRT complex / viral nucleocapsid / host cell cytoplasm / ribonucleoprotein complex / RNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Taylor, A.B. / Garza, J.A. | ||||||
![]() | ![]() Title: Unveiling a Drift Resistant Cryptotope withinMarburgvirusNucleoprotein Recognized by Llama Single-Domain Antibodies. Authors: Garza, J.A. / Taylor, A.B. / Sherwood, L.J. / Hart, P.J. / Hayhurst, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 154.9 KB | Display | ![]() |
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PDB format | ![]() | 121.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 498.6 KB | Display | ![]() |
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Full document | ![]() | 500.9 KB | Display | |
Data in XML | ![]() | 25.8 KB | Display | |
Data in CIF | ![]() | 34.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4w2pC ![]() 4w2qC ![]() 6apoC ![]() 6appSC ![]() 6apqC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 13758.283 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: Semi-synthetic single pot library Nomad 1 based upon Lama glama Plasmid: pecan73 / Production host: ![]() ![]() #2: Protein | Mass: 12238.336 Da / Num. of mol.: 4 / Fragment: C-terminal domain residues 601-695 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: Musoke-80 / Gene: NP / Plasmid: pE-NP600 / Production host: ![]() ![]() #3: Chemical | ChemComp-SO4 / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.53 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop Details: 20% polyethylene glycol 4000, 0.16M ammonium sulfate, 20% glycerol, 0.08M sodium acetate pH 4.6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: NOIR-1 / Detector: CCD / Date: May 23, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97626 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→58 Å / Num. obs: 15378 / % possible obs: 100 % / Redundancy: 7.2 % / Biso Wilson estimate: 55.1 Å2 / Rpim(I) all: 0.081 / Rsym value: 0.189 / Net I/σ(I): 10.6 |
Reflection shell | Resolution: 3.2→3.37 Å / Redundancy: 7.4 % / Mean I/σ(I) obs: 3 / Num. unique obs: 2189 / Rpim(I) all: 0.297 / Rsym value: 0.705 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6APP Resolution: 3.2→54.328 Å / SU ML: 0.45 / Cross valid method: THROUGHOUT / σ(F): 1.94 / Phase error: 30.32
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Solvent computation | Shrinkage radii: 0.8 Å / VDW probe radii: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 51.3 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.2→54.328 Å
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Refine LS restraints |
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LS refinement shell |
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