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Yorodumi- PDB-1sl6: Crystal Structure of a fragment of DC-SIGNR (containg the carbohy... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1sl6 | |||||||||
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Title | Crystal Structure of a fragment of DC-SIGNR (containg the carbohydrate recognition domain and two repeats of the neck) complexed with Lewis-x. | |||||||||
Components | C-type lectin DC-SIGNR | |||||||||
Keywords | SUGAR BINDING PROTEIN / DC-SIGNR / C-TYPE LECTIN | |||||||||
Function / homology | Function and homology information cell-cell recognition / intracellular transport of virus / peptide antigen transport / ICAM-3 receptor activity / fucose binding / virion binding / leukocyte cell-cell adhesion / RSV-host interactions / antigen processing and presentation / D-mannose binding ...cell-cell recognition / intracellular transport of virus / peptide antigen transport / ICAM-3 receptor activity / fucose binding / virion binding / leukocyte cell-cell adhesion / RSV-host interactions / antigen processing and presentation / D-mannose binding / viral genome replication / peptide antigen binding / calcium-dependent protein binding / host cell / virus receptor activity / signaling receptor activity / carbohydrate binding / adaptive immune response / receptor-mediated endocytosis of virus by host cell / receptor-mediated virion attachment to host cell / intracellular signal transduction / symbiont entry into host cell / immune response / external side of plasma membrane / innate immune response / virion attachment to host cell / extracellular region / membrane / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.25 Å | |||||||||
Authors | Guo, Y. / Feinberg, H. / Conroy, E. / Mitchell, D.A. / Alvarez, R. / Blixt, O. / Taylor, M.E. / Weis, W.I. / Drickamer, K. | |||||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2004 Title: Structural basis for distinct ligand-binding and targeting properties of the receptors DC-SIGN and DC-SIGNR Authors: Guo, Y. / Feinberg, H. / Conroy, E. / Mitchell, D.A. / Alvarez, R. / Blixt, O. / Taylor, M.E. / Weis, W.I. / Drickamer, K. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sl6.cif.gz | 226.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sl6.ent.gz | 185.2 KB | Display | PDB format |
PDBx/mmJSON format | 1sl6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sl6_validation.pdf.gz | 2.7 MB | Display | wwPDB validaton report |
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Full document | 1sl6_full_validation.pdf.gz | 2.7 MB | Display | |
Data in XML | 1sl6_validation.xml.gz | 41.1 KB | Display | |
Data in CIF | 1sl6_validation.cif.gz | 56.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sl/1sl6 ftp://data.pdbj.org/pub/pdb/validation_reports/sl/1sl6 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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6 |
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Unit cell |
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-Components
#1: Protein | Mass: 21404.506 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Details: A fragment containing the CRD domain and two repeats from the neck domain Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): BL21/DE3 / Production host: Escherichia coli (E. coli) / References: GenBank: 12084795, UniProt: Q9H2X3*PLUS #2: Polysaccharide | alpha-L-fucopyranose-(1-3)-[beta-D-galactopyranose-(1-4)]2-acetamido-2-deoxy-alpha-D-glucopyranose / Lewis X antigen / alpha anomer #3: Chemical | ChemComp-CA / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.69 Å3/Da / Density % sol: 66.37 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 30% PEG 300, 0.2M NaCl, 0.1 Hepes pH=7.5. Protein solution: 10 mg/ml protein, 5mM CaCl2, 10 mM oligosaccharide., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1.0781 Å |
Detector | Type: ADSC / Detector: CCD / Date: Jul 3, 2003 |
Radiation | Monochromator: Double-crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0781 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→50 Å / Num. all: 83241 / Num. obs: 80306 / Observed criterion σ(I): -3 / Biso Wilson estimate: 25.9 Å2 / Rsym value: 0.054 |
Reflection shell | Resolution: 2.25→2.33 Å / Rsym value: 0.322 / % possible all: 84.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.25→41.96 Å / Rfactor Rfree error: 0.003 / Data cutoff high absF: 3723247.21 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 38.8621 Å2 / ksol: 0.348332 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 54.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.25→41.96 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.25→2.39 Å / Rfactor Rfree error: 0.013 / Total num. of bins used: 6
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Xplor file |
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