|Entry||Database: PDB / ID: 5d5b|
|Title||In meso X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K|
|Components||Beta-2 adrenergic receptor,Endolysin,Beta-2 adrenergic receptor|
|Keywords||Membrane Protein/Hydrolase / MEMBRANE PROTEIN / HYDROLASE / Membrane Protein-Hydrolase complex|
|Function / homology|
Function and homology information
beta2-adrenergic receptor activity / desensitization of G protein-coupled receptor signaling pathway by arrestin / positive regulation of mini excitatory postsynaptic potential / norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure / positive regulation of cAMP-dependent protein kinase activity / Adrenoceptors / norepinephrine binding / positive regulation of autophagosome maturation / heat generation / activation of transmembrane receptor protein tyrosine kinase activity ...beta2-adrenergic receptor activity / desensitization of G protein-coupled receptor signaling pathway by arrestin / positive regulation of mini excitatory postsynaptic potential / norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure / positive regulation of cAMP-dependent protein kinase activity / Adrenoceptors / norepinephrine binding / positive regulation of autophagosome maturation / heat generation / activation of transmembrane receptor protein tyrosine kinase activity / positive regulation of lipophagy / response to psychosocial stress / negative regulation of smooth muscle contraction / negative regulation of multicellular organism growth / positive regulation of AMPA receptor activity / diet induced thermogenesis / adrenergic receptor signaling pathway / endosome to lysosome transport / positive regulation of bone mineralization / brown fat cell differentiation / adenylate cyclase-activating adrenergic receptor signaling pathway / positive regulation of protein kinase A signaling / potassium channel regulator activity / adenylate cyclase binding / activation of adenylate cyclase activity / bone resorption / regulation of sodium ion transport / response to cold / receptor-mediated endocytosis / G protein-coupled receptor activity / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / viral release from host cell by cytolysis / ADORA2B mediated anti-inflammatory cytokines production / positive regulation of protein serine/threonine kinase activity / clathrin-coated endocytic vesicle membrane / peptidoglycan catabolic process / G alpha (s) signalling events / cellular response to amyloid-beta / positive regulation of cold-induced thermogenesis / Cargo recognition for clathrin-mediated endocytosis / Clathrin-mediated endocytosis / cell wall macromolecule catabolic process / amyloid-beta binding / lysozyme / lysozyme activity / cytolysis / positive regulation of MAPK cascade / apical plasma membrane / lysosome / endosome membrane / cell surface receptor signaling pathway / early endosome / receptor complex / Ub-specific processing proteases / endosome / host cell cytoplasm / defense response to bacterium / protein-containing complex binding / Golgi apparatus / integral component of plasma membrane / positive regulation of transcription by RNA polymerase II / protein homodimerization activity / membrane / identical protein binding / plasma membrane / nucleus
Similarity search - Function
Beta 2 adrenoceptor / Adrenoceptor family / Serpentine type 7TM GPCR chemoreceptor Srsx / Endolysin T4 type / T4-type lysozyme / G-protein coupled receptors family 1 signature. / Glycoside hydrolase, family 24 / Lysozyme domain superfamily / Phage lysozyme / 7 transmembrane receptor (rhodopsin family) ...Beta 2 adrenoceptor / Adrenoceptor family / Serpentine type 7TM GPCR chemoreceptor Srsx / Endolysin T4 type / T4-type lysozyme / G-protein coupled receptors family 1 signature. / Glycoside hydrolase, family 24 / Lysozyme domain superfamily / Phage lysozyme / 7 transmembrane receptor (rhodopsin family) / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / Lysozyme-like domain superfamily
Similarity search - Domain/homology
Endolysin / beta-maltose / ACETAMIDE / PALMITIC ACID / CHOLESTEROL / Chem-CAU / 1,4-BUTANEDIOL / Beta-2 adrenergic receptor
Similarity search - Component
|Biological species||Homo sapiens (human)|
Enterobacteria phage T4 (bacteriophage)
|Method||X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.8 Å|
|Authors||Huang, C.-Y. / Olieric, V. / Liu, X. / Kobilka, B. / Wang, M. / Caffrey, M.|
|Funding support|| Ireland, 1items |
|Citation||Journal: Acta Crystallogr D Struct Biol / Year: 2016|
Title: In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
Authors: Huang, C.Y. / Olieric, V. / Ma, P. / Howe, N. / Vogeley, L. / Liu, X. / Warshamanage, R. / Weinert, T. / Panepucci, E. / Kobilka, B. / Diederichs, K. / Wang, M. / Caffrey, M.
|Structure viewer||Molecule: |
Downloads & links
A: Beta-2 adrenergic receptor,Endolysin,Beta-2 adrenergic receptor
-Protein / Sugars , 2 types, 2 molecules A
-Non-polymers , 6 types, 13 molecules
|#3: Chemical|| ChemComp-CAU / (|
|#4: Chemical|| ChemComp-BU1 / |
|#5: Chemical|| ChemComp-ACM / |
|#6: Chemical||#7: Chemical|| ChemComp-PLM / ||#8: Chemical|
|Experiment||Method: X-RAY DIFFRACTION|
|Crystal||Density Matthews: 4.02 Å3/Da / Density % sol: 69.42 %|
|Crystal grow||Temperature: 293 K / Method: lipidic cubic phase|
Details: 30-35 %(v/v) PEG 400, 0.1-0.2 M Na2SO4, 0.1 M bis-tris propane pH 6.5-7.0 and 5-7 %(v/v) 1,4-butanediol
|Diffraction||Mean temperature: 100 K|
|Diffraction source||Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1.03321 Å|
|Detector||Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Feb 18, 2015|
|Radiation||Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray|
|Radiation wavelength||Wavelength: 1.03321 Å / Relative weight: 1|
|Reflection||Resolution: 3.8→50 Å / Num. obs: 6835 / % possible obs: 99.6 % / Redundancy: 6.5 % / Net I/σ(I): 3.76|
|Reflection shell||Resolution: 3.8→3.9 Å / Redundancy: 4.58 % / Mean I/σ(I) obs: 0.96 / % possible all: 98.8|
|Refinement||Method to determine structure: MOLECULAR REPLACEMENT|
Starting model: 2rh1
Resolution: 3.8→49.618 Å / SU ML: 0.52 / Cross valid method: THROUGHOUT / σ(F): 1.37 / Phase error: 33.87 / Stereochemistry target values: ML
|Solvent computation||Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL|
|Refinement step||Cycle: LAST / Resolution: 3.8→49.618 Å|
|Refine LS restraints|
|LS refinement shell|
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