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Structure paper

TitleIn meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
Journal, issue, pagesActa Crystallogr D Struct Biol, Vol. 72, Page 93-112, Year 2016
Publish dateAug 10, 2015 (structure data deposition date)
AuthorsHuang, C.Y. / Olieric, V. / Ma, P. / Howe, N. / Vogeley, L. / Liu, X. / Warshamanage, R. / Weinert, T. / Panepucci, E. / Kobilka, B. ...Huang, C.Y. / Olieric, V. / Ma, P. / Howe, N. / Vogeley, L. / Liu, X. / Warshamanage, R. / Weinert, T. / Panepucci, E. / Kobilka, B. / Diederichs, K. / Wang, M. / Caffrey, M.
External linksActa Crystallogr D Struct Biol / PubMed:26894538
MethodsX-ray diffraction
Resolution1.5 - 3.8 Å
Structure data

PDB-5d52:
In meso in situ serial X-ray crystallography structure of insulin at room temperature
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-5d53:
In meso in situ serial X-ray crystallography structure of insulin at 100 K
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5d54:
In meso X-ray crystallography structure of insulin at 100 K
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-5d56:
In meso in situ serial X-ray crystallography structure of diacylglycerol kinase, DgkA, at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-5d57:
In meso X-ray crystallography structure of diacylglycerol kinase, DgkA, at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-5d58:
In meso in situ serial X-ray crystallography structure of the PepTSt-Ala-Phe complex at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5d59:
In meso X-ray crystallography structure of the PepTSt-Ala-Phe complex at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5d5a:
In meso in situ serial X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2.4826 Å

PDB-5d5b:
In meso X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K
Method: X-RAY DIFFRACTION / Resolution: 3.8 Å

PDB-5d5c:
In meso in situ serial X-ray crystallography structure of lysozyme at 100 K
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-5d5d:
In meso in situ serial X-ray crystallography structure of AlgE at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-5d5e:
In meso in situ serial X-ray crystallography structure of insulin by sulfur-SAD at 100 K
Method: X-RAY DIFFRACTION / Resolution: 2.407 Å

PDB-5d5f:
In meso in situ serial X-ray crystallography structure of lysozyme by bromine-SAD at 100 K
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-HOH:
WATER / Water

ChemComp-PE5:
3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL / precipitant*YM / Polyethylene glycol

ChemComp-78M:
(2S)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE

ChemComp-ZN:
Unknown entry

ChemComp-FLC:
CITRATE ANION / Citric acid

ChemComp-ACT:
ACETATE ION / Acetate

ChemComp-ALA:
ALANINE / Alanine

ChemComp-PHE:
PHENYLALANINE / Phenylalanine

ChemComp-CAU:
(2S)-1-(9H-Carbazol-4-yloxy)-3-(isopropylamino)propan-2-ol

ChemComp-BU1:
1,4-BUTANEDIOL / 1,4-Butanediol

ChemComp-ACM:
ACETAMIDE / Acetamide

ChemComp-CLR:
CHOLESTEROL / Cholesterol

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

ChemComp-12P:
DODECAETHYLENE GLYCOL / precipitant*YM / Polyethylene glycol

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-BR:
BROMIDE ION / Bromide

ChemComp-NA:
Unknown entry

ChemComp-ACY:
ACETIC ACID / Acetic acid

ChemComp-CA:
Unknown entry

ChemComp-LDA:
LAURYL DIMETHYLAMINE-N-OXIDE / LDAO, detergent*YM / Lauryldimethylamine oxide

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM / MES (buffer)

Source
  • sus scrofa (pig)
  • escherichia coli k-12 (bacteria)
  • streptococcus thermophilus (strain atcc baa-250 / lmg 18311) (bacteria)
  • synthetic construct (others)
  • homo sapiens (human)
  • enterobacteria phage t4 (virus)
  • gallus gallus (chicken)
  • pseudomonas aeruginosa (strain atcc 15692 / pao1 / 1c / prs 101 / lmg 12228) (bacteria)
KeywordsHORMONE / TRANSFERASE / TRANSPORT PROTEIN / Membrane Protein/Hydrolase / MEMBRANE PROTEIN / HYDROLASE / Membrane Protein-Hydrolase complex / Beta-Barrel Membrane Proteins / AlgE alginate export protein

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