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- PDB-5wf6: Agonist bound human A2a adenosine receptor with S91A mutation at ... -

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Basic information

Entry
Database: PDB / ID: 5wf6
TitleAgonist bound human A2a adenosine receptor with S91A mutation at 2.90 A resolution
ComponentsHuman A2a adenosine receptor T4L chimera
KeywordsMEMBRANE PROTEIN / human A2a adenosine receptor / S91A / GPCR-T4L chimera / LCP / agonist / UK432097 / Activation microswitch / allosteric Na-site mutant / GPCR signaling
Function / homology
Function and homology information


positive regulation of acetylcholine secretion, neurotransmission / Adenosine P1 receptors / positive regulation of circadian sleep/wake cycle, sleep / G protein-coupled adenosine receptor activity / negative regulation of alpha-beta T cell activation / sensory perception / regulation of norepinephrine secretion / negative regulation of locomotion / synaptic transmission, dopaminergic / response to purine-containing compound ...positive regulation of acetylcholine secretion, neurotransmission / Adenosine P1 receptors / positive regulation of circadian sleep/wake cycle, sleep / G protein-coupled adenosine receptor activity / negative regulation of alpha-beta T cell activation / sensory perception / regulation of norepinephrine secretion / negative regulation of locomotion / synaptic transmission, dopaminergic / response to purine-containing compound / positive regulation of urine volume / Surfactant metabolism / NGF-independant TRKA activation / biomineralization / positive regulation of renal sodium excretion / response to caffeine / inhibitory postsynaptic potential / protein kinase C-activating G protein-coupled receptor signaling pathway / blood circulation / response to inorganic substance / synaptic transmission, cholinergic / type 5 metabotropic glutamate receptor binding / positive regulation of glutamate secretion / negative regulation of vascular permeability / intermediate filament / eating behavior / membrane depolarization / presynaptic active zone / axolemma / alpha-actinin binding / synaptic transmission, glutamatergic / regulation of calcium ion transport / positive regulation of apoptotic signaling pathway / positive regulation of synaptic transmission, glutamatergic / phagocytosis / endomembrane system / cellular defense response / excitatory postsynaptic potential / prepulse inhibition / astrocyte activation / neuron projection morphogenesis / response to amphetamine / integral component of postsynaptic membrane / viral release from host cell by cytolysis / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / locomotory behavior / regulation of mitochondrial membrane potential / adenylate cyclase-activating G protein-coupled receptor signaling pathway / ADORA2B mediated anti-inflammatory cytokines production / regulation of synaptic vesicle exocytosis / positive regulation of long-term synaptic potentiation / peptidoglycan catabolic process / central nervous system development / positive regulation of synaptic transmission, GABAergic / integral component of presynaptic membrane / positive regulation of protein secretion / G alpha (s) signalling events / vasodilation / negative regulation of protein kinase activity / negative regulation of inflammatory response / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / cell-cell signaling / blood coagulation / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / cytolysis / negative regulation of neuron apoptotic process / postsynaptic density / response to xenobiotic stimulus / inflammatory response / negative regulation of cell population proliferation / host cell cytoplasm / defense response to bacterium / glutamatergic synapse / neuronal cell body / dendrite / apoptotic process / protein-containing complex binding / regulation of DNA-templated transcription / enzyme binding / integral component of plasma membrane / membrane / identical protein binding / plasma membrane
Similarity search - Function
Adenosine A2A receptor / Adenosine receptor / Serpentine type 7TM GPCR chemoreceptor Srsx / Endolysin T4 type / T4-type lysozyme / Lysozyme domain superfamily / Glycoside hydrolase, family 24 / Phage lysozyme / G-protein coupled receptors family 1 signature. / 7 transmembrane receptor (rhodopsin family) ...Adenosine A2A receptor / Adenosine receptor / Serpentine type 7TM GPCR chemoreceptor Srsx / Endolysin T4 type / T4-type lysozyme / Lysozyme domain superfamily / Glycoside hydrolase, family 24 / Phage lysozyme / G-protein coupled receptors family 1 signature. / 7 transmembrane receptor (rhodopsin family) / G-protein coupled receptors family 1 profile. / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / Lysozyme-like domain superfamily
Similarity search - Domain/homology
Endolysin / (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate / Chem-UKA / Adenosine receptor A2a
Similarity search - Component
Biological speciesHomo sapiens (human)
Enterobacteria phage T4 (bacteriophage)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.9 Å
AuthorsWhite, K.L. / Eddy, M.T. / Gao, Z. / Han, G.W. / Hanson, M.A. / Lian, T. / Deary, A. / Patel, N. / Jacobson, K.A. / Katritch, V. / Stevens, R.C.
CitationJournal: Structure / Year: 2018
Title: Structural Connection between Activation Microswitch and Allosteric Sodium Site in GPCR Signaling.
Authors: White, K.L. / Eddy, M.T. / Gao, Z.G. / Han, G.W. / Lian, T. / Deary, A. / Patel, N. / Jacobson, K.A. / Katritch, V. / Stevens, R.C.
History
DepositionJul 11, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 21, 2018Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
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Assembly

Deposited unit
A: Human A2a adenosine receptor T4L chimera
hetero molecules


Theoretical massNumber of molelcules
Total (without water)57,6743
Polymers56,5401
Non-polymers1,1342
Water362
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area0 Å2
ΔGint0 kcal/mol
Surface area21420 Å2
MethodPISA
Unit cell
Length a, b, c (Å)47.783, 78.226, 86.477
Angle α, β, γ (deg.)90.00, 101.20, 90.00
Int Tables number4
Space group name H-MP1211
DetailsAUTHORS STATE THAT THE BIOLOGICAL UNIT IS UNKNOWN

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Components

#1: Protein Human A2a adenosine receptor T4L chimera / Lysis protein / Lysozyme / Muramidase


Mass: 56540.059 Da / Num. of mol.: 1 / Mutation: R1012G, C1054T, C1097A, I1137R
Source method: isolated from a genetically manipulated source
Details: S91A mutation
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Enterobacteria phage T4 (bacteriophage)
Gene: ADORA2A, ADORA2 / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): sf9 / References: UniProt: P29274, UniProt: P00720, lysozyme
#2: Chemical ChemComp-UKA / 6-(2,2-diphenylethylamino)-9-[(2R,3R,4S,5S)-5-(ethylcarbamoyl)-3,4-dihydroxy-oxolan-2-yl]-N-[2-[(1-pyridin-2-ylpiperidin-4-yl)carbamoylamino]ethyl]purine-2-carboxamide / UK-432,097


Mass: 777.871 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C40H47N11O6 / Comment: agonist*YM
#3: Chemical ChemComp-OLC / (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate / 1-Oleoyl-R-glycerol


Mass: 356.540 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C21H40O4 / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.9 Å3/Da / Density % sol: 57.54 %
Crystal growTemperature: 293 K / Method: lipidic cubic phase
Details: 100 mM sodium citrate pH 5, 24-27% (v/v) polyethylene glycol (PEG) 400, 30-80 mM MgCl 2 , 5% (v/v) Jeffamine M-600 pH 7 (Hampton)

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1.033 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 18, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.033 Å / Relative weight: 1
ReflectionResolution: 2.9→44.88 Å / Num. obs: 13000 / % possible obs: 91.9 % / Redundancy: 3.3 % / Rmerge(I) obs: 0.218 / Net I/σ(I): 4.6
Reflection shellResolution: 2.9→3 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.71 / Mean I/σ(I) obs: 1.3 / % possible all: 74.5

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Processing

Software
NameVersionClassification
PHENIX(dev_2614: ???)refinement
DENZOdata reduction
SCALEPACKdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB 3EML
Resolution: 2.9→29.487 Å / SU ML: 0.43 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 37.15
RfactorNum. reflection% reflection
Rfree0.2877 660 5.14 %
Rwork0.2525 --
obs0.2544 12850 91.84 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.9→29.487 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3405 0 66 2 3473
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0033554
X-RAY DIFFRACTIONf_angle_d0.4834844
X-RAY DIFFRACTIONf_dihedral_angle_d14.3692110
X-RAY DIFFRACTIONf_chiral_restr0.037572
X-RAY DIFFRACTIONf_plane_restr0.003632
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.9002-3.12390.3387950.33582073X-RAY DIFFRACTION78
3.1239-3.43780.3761350.30252454X-RAY DIFFRACTION93
3.4378-3.93430.29831300.2672557X-RAY DIFFRACTION97
3.9343-4.9530.2981510.25472510X-RAY DIFFRACTION96
4.953-29.48850.261490.22752596X-RAY DIFFRACTION96

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