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Yorodumi- PDB-4n6s: Crystals of cross-linked stabilized and functional Phycobilisomes... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4n6s | ||||||
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Title | Crystals of cross-linked stabilized and functional Phycobilisomes: only phycocyanin rods contribute to diffraction. | ||||||
Components |
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Keywords | PHOTOSYNTHESIS / antenna / glutaraldehyde cross-links / membrane associated | ||||||
Function / homology | Function and homology information phycobilisome / plasma membrane-derived thylakoid membrane / photosynthesis Similarity search - Function | ||||||
Biological species | Thermosynechococcus vulcanus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | David, L. / Prado, M. / Arteni, A. / Elmlund, D.A. / Blankenship, R.E. / Adir, N. | ||||||
Citation | Journal: Biochim.Biophys.Acta / Year: 2014 Title: Structural studies show energy transfer within stabilized phycobilisomes independent of the mode of rod-core assembly. Authors: David, L. / Prado, M. / Arteni, A.A. / Elmlund, D.A. / Blankenship, R.E. / Adir, N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4n6s.cif.gz | 81.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4n6s.ent.gz | 60.8 KB | Display | PDB format |
PDBx/mmJSON format | 4n6s.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n6/4n6s ftp://data.pdbj.org/pub/pdb/validation_reports/n6/4n6s | HTTPS FTP |
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-Related structure data
Related structure data | 3o18S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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Details | THE AUTHOR STATES THAT THE BIOLOGICAL UNIT OF THIS PROTEIN IS UNKNOWN. |
-Components
#1: Protein | Mass: 17470.656 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermosynechococcus vulcanus (bacteria) / References: UniProt: Q9AM02 | ||
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#2: Protein | Mass: 18216.652 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Thermosynechococcus vulcanus (bacteria) / References: UniProt: Q71RW8 | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.28 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 10-15 mg/ml phycobilisome in 0.9M phosphate buffer against a resevoir of 1.2-1.4M phosphate buffer. , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.9795 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 1, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→54.3 Å / Num. obs: 16012 / % possible obs: 99.9 % / Redundancy: 4.1 % / Rmerge(I) obs: 0.071 / Net I/σ(I): 20 |
Reflection shell | Resolution: 2.4→2.53 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.312 / Mean I/σ(I) obs: 6.3 / Num. unique all: 2307 / % possible all: 98.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3O18 Resolution: 2.4→50 Å / Cross valid method: THROUGHOUT / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 31.13 Å2 | |||||||||||||||||||||||||
Refine analyze | Luzzati d res low obs: 5 Å / Luzzati sigma a obs: 0.284 Å | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→50 Å
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Refine LS restraints |
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