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Open data
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Basic information
Entry | Database: PDB / ID: 2cdt | ||||||
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Title | alpha-SPECTRIN SH3 DOMAIN A56S MUTANT | ||||||
![]() | SPECTRIN ALPHA CHAIN | ||||||
![]() | STRUCTURAL PROTEIN / SH3-DOMAIN / CYTOSKELETON / CALMODULIN-BINDING / ACTIN-BINDING | ||||||
Function / homology | ![]() actin filament capping / costamere / cortical actin cytoskeleton / cell projection / actin filament binding / cell junction / actin cytoskeleton organization / calmodulin binding / calcium ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Casares, S. / Camara-Artigas, A. / Vega, M.C. / Lopez-Mayorga, O. / Conejero-Lara, F. | ||||||
![]() | ![]() Title: Cooperative Propagation of Local Stability Changes from Low-Stability and High-Stability Regions in a SH3 Domain. Authors: Casares, S. / Lopez-Mayorga, O. / Vega, M.C. / Camara-Artigas, A. / Conejero-Lara, F. #1: ![]() Title: Crystal Structure of a Src-Homology 3 (SH3) Domain Authors: Musacchio, A. / Noble, M. / Pauptit, R. / Wierenga, R. / Saraste, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 24.2 KB | Display | ![]() |
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PDB format | ![]() | 15.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 417.2 KB | Display | ![]() |
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Full document | ![]() | 418.4 KB | Display | |
Data in XML | ![]() | 4.5 KB | Display | |
Data in CIF | ![]() | 5.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2f2vC ![]() 2f2wC ![]() 2f2xC ![]() 1h1g S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 7245.244 Da / Num. of mol.: 1 / Fragment: SH3-DOMAIN, RESIDUES 969-1025 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
Compound details | ENGINEERED |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 53.2 % |
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Crystal grow | pH: 6 Details: 1.1M AMMONIUM SULPHATE, 90MM SODIUM CITRATE/CITRIC ACID, PH 6.0, 90 MM BIS-TRIS PROPANE, 0.9MM EDTA, 0.9MM DTT, 0.9MM SODIUM AZIDE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 15, 2000 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.45→15 Å / Num. obs: 2316 / % possible obs: 89.3 % / Observed criterion σ(I): 2 / Redundancy: 4.5 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 6.2 |
Reflection shell | Resolution: 2.45→2.6 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.14 / Mean I/σ(I) obs: 3.6 / % possible all: 97.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1H1G ![]() 1h1g Resolution: 2.54→14.92 Å / Cor.coef. Fo:Fc: 0.902 / Cor.coef. Fo:Fc free: 0.838 / Cross valid method: THROUGHOUT / ESU R: 0.743 / ESU R Free: 0.351 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.4 Å2
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Refinement step | Cycle: LAST / Resolution: 2.54→14.92 Å
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Refine LS restraints |
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