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Open data
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Basic information
Entry | Database: PDB / ID: 1hd3 | ||||||
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Title | A-SPECTRIN SH3 DOMAIN F52Y MUTANT | ||||||
![]() | SPECTRIN ALPHA CHAIN | ||||||
![]() | CYTOSKELETON / SH3-DOMAIN / CALMODULIN-BINDING / ACTIN-BINDING | ||||||
Function / homology | ![]() actin filament capping / costamere / cortical actin cytoskeleton / cell projection / actin filament binding / cell junction / actin cytoskeleton organization / calmodulin binding / calcium ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Vega, M.C. / Viguera, A.R. / Serrano, L. | ||||||
![]() | ![]() Title: Unspecific Hydrophobic Stabilization of Folding Transition States Authors: Viguera, A.R. / Vega, C. / Serrano, L. #1: ![]() Title: Crystal Structure of a Src-Homology 3 (SH3) Domain Authors: Musacchio, A. / Noble, M. / Pauptit, R. / Wierenga, R. / Saraste, M. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: DSSP | ||||||
Remark 700 | SHEET DETERMINATION METHOD: DSSP |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 27.3 KB | Display | ![]() |
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PDB format | ![]() | 17 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 389.2 KB | Display | ![]() |
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Full document | ![]() | 391 KB | Display | |
Data in XML | ![]() | 3.3 KB | Display | |
Data in CIF | ![]() | 4.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1shgS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | BIOLOGICAL_UNIT: MONOMER |
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Components
#1: Protein | Mass: 7245.244 Da / Num. of mol.: 1 / Fragment: SH3-DOMAIN RESIDUES 964-1025 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||||
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#2: Chemical | ChemComp-SO4 / | ||||||
#3: Chemical | #4: Water | ChemComp-HOH / | Compound details | CHAIN A ENGINEERED | Sequence details | 1SHG SWS P07751 1 - 968 NOT IN ATOMS LIST 1SHG SWS P07751 1026 - 2477 NOT IN ATOMS LIST | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.56 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6 Details: PROTEIN WAS CRYSTALLIZED FROM 1.1 M AMMONIUM SULPHATE, 90MM SODIUM CITRATE/CITRIC ACID, PH=6.0, 90 MM BIS-TRIS PROPANE, 0.9 MM EDTA, 0.9 MM DTT, 0.9 MM SODIUM AZIDE, pH 6.00 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 3.5 / Method: vapor diffusion, hanging drop / Details: Musacchio, A., (1992) Nature, 359, 851. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH IMAGING PLATE / Date: Aug 15, 1999 / Details: MIRROR |
Radiation | Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.05 Å / Relative weight: 1 |
Reflection | Resolution: 1.98→8 Å / Num. obs: 3987 / % possible obs: 87.4 % / Observed criterion σ(I): 2 / Redundancy: 2.3 % / Rmerge(I) obs: 0.119 |
Reflection shell | Resolution: 1.98→2.07 Å / Rmerge(I) obs: 0.09 / % possible all: 75 |
Reflection | *PLUS Rmerge(I) obs: 0.09 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1SHG Resolution: 1.98→8 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.98→8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.98→2.07 Å / % reflection obs: 75 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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