+Open data
-Basic information
Entry | Database: PDB / ID: 1bk2 | ||||||
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Title | A-SPECTRIN SH3 DOMAIN D48G MUTANT | ||||||
Components | A-SPECTRIN | ||||||
Keywords | SH3-DOMAIN / CYTOSKELETON / CALMODULIN-BINDING / ACTIN-BINDING | ||||||
Function / homology | Function and homology information costamere / actin filament capping / cortical actin cytoskeleton / cell projection / actin filament binding / cell junction / actin cytoskeleton organization / calmodulin binding / calcium ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.01 Å | ||||||
Authors | Martinez, J.C. / Pisabarro, M.T. / Serrano, L. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1998 Title: Obligatory steps in protein folding and the conformational diversity of the transition state. Authors: Martinez, J.C. / Pisabarro, M.T. / Serrano, L. #1: Journal: Nature / Year: 1992 Title: Crystal Structure of a Src-Homology 3 (SH3) Domain Authors: Musacchio, A. / Noble, M. / Pauptit, R. / Wierenga, R. / Saraste, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bk2.cif.gz | 21.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bk2.ent.gz | 14.6 KB | Display | PDB format |
PDBx/mmJSON format | 1bk2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bk/1bk2 ftp://data.pdbj.org/pub/pdb/validation_reports/bk/1bk2 | HTTPS FTP |
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-Related structure data
Related structure data | 1shgS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 6637.656 Da / Num. of mol.: 1 / Fragment: SH3-DOMAIN / Mutation: D48G Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Cell line: BL21 / Plasmid: PBR322 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P07751 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.34 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6 / Details: pH 6.0 | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: MACSCIENCE / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 1, 1998 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→15 Å / Num. obs: 47630 / % possible obs: 96.5 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.044 |
Reflection shell | Resolution: 2→2.1 Å / Rmerge(I) obs: 0.121 / % possible all: 95.5 |
Reflection | *PLUS Num. obs: 4758 / Num. measured all: 47630 |
Reflection shell | *PLUS % possible obs: 95.5 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1SHG Resolution: 2.01→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 35.29 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.01→8 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.8 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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