+Open data
-Basic information
Entry | Database: PDB / ID: 1oeo | ||||||
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Title | PTP1B with the catalytic cysteine oxidized to sulfonic acid | ||||||
Components | PROTEIN-TYROSINE PHOSPHATASE, NON-RECEPTOR TYPE 1 | ||||||
Keywords | HYDROLASE / PHOSPHORYLATION / CYSTEINE SULFONIC ACID | ||||||
Function / homology | Function and homology information regulation of hepatocyte growth factor receptor signaling pathway / PTK6 Down-Regulation / peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity / positive regulation of receptor catabolic process / insulin receptor recycling / negative regulation of PERK-mediated unfolded protein response / negative regulation of vascular endothelial growth factor receptor signaling pathway / regulation of intracellular protein transport / IRE1-mediated unfolded protein response / cytoplasmic side of endoplasmic reticulum membrane ...regulation of hepatocyte growth factor receptor signaling pathway / PTK6 Down-Regulation / peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity / positive regulation of receptor catabolic process / insulin receptor recycling / negative regulation of PERK-mediated unfolded protein response / negative regulation of vascular endothelial growth factor receptor signaling pathway / regulation of intracellular protein transport / IRE1-mediated unfolded protein response / cytoplasmic side of endoplasmic reticulum membrane / sorting endosome / mitochondrial crista / platelet-derived growth factor receptor-beta signaling pathway / positive regulation of IRE1-mediated unfolded protein response / regulation of type I interferon-mediated signaling pathway / regulation of endocytosis / non-membrane spanning protein tyrosine phosphatase activity / peptidyl-tyrosine dephosphorylation / Regulation of IFNA/IFNB signaling / regulation of signal transduction / cellular response to unfolded protein / positive regulation of protein tyrosine kinase activity / growth hormone receptor signaling pathway via JAK-STAT / negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / negative regulation of signal transduction / Regulation of IFNG signaling / MECP2 regulates neuronal receptors and channels / endoplasmic reticulum unfolded protein response / Growth hormone receptor signaling / positive regulation of JUN kinase activity / negative regulation of insulin receptor signaling pathway / Insulin receptor recycling / ephrin receptor binding / Integrin signaling / protein dephosphorylation / negative regulation of MAP kinase activity / protein-tyrosine-phosphatase / protein phosphatase 2A binding / protein tyrosine phosphatase activity / endosome lumen / insulin receptor binding / Negative regulation of MET activity / negative regulation of ERK1 and ERK2 cascade / receptor tyrosine kinase binding / insulin receptor signaling pathway / actin cytoskeleton organization / early endosome / mitochondrial matrix / cadherin binding / protein kinase binding / enzyme binding / endoplasmic reticulum / protein-containing complex / RNA binding / zinc ion binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.15 Å | ||||||
Authors | Salmeen, A. / Andersen, J.N. / Myers, M.P. / Meng, T.C. / Hinks, J.A. / Tonks, N.K. / Barford, D. | ||||||
Citation | Journal: Nature / Year: 2003 Title: Redox Regulation of Protein Tyrosine Phosphatase Involves a Sulfenyl-Amide Intermediate Authors: Salmeen, A. / Andersen, J.N. / Myers, M.P. / Meng, T.C. / Hinks, J.A. / Tonks, N.K. / Barford, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1oeo.cif.gz | 75.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1oeo.ent.gz | 54.9 KB | Display | PDB format |
PDBx/mmJSON format | 1oeo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oe/1oeo ftp://data.pdbj.org/pub/pdb/validation_reports/oe/1oeo | HTTPS FTP |
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-Related structure data
Related structure data | 1oemC 2hnpS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 37413.637 Da / Num. of mol.: 1 / Fragment: CATALYTIC DOMAIN, RESIDUES 1-321 Source method: isolated from a genetically manipulated source Details: CATALYTIC CYSTEINE X215 IS OXIDIZED TO A SULFONIC ACID Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: P18031, protein-tyrosine-phosphatase |
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#2: Water | ChemComp-HOH / |
Compound details | CATALYSES HYDROLYSIS |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.27 Å3/Da / Density % sol: 62.09 % | |||||||||||||||||||||||||
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Crystal grow | pH: 7.5 Details: 0.1M HEPES PH 7.5, 12%PEG, 0.2M MGCL2,CRYSTALS WERE SOAKED OVERNIGHT IN 100 MICROMOLAR PERVANADATE PRIOR TO DATA COLLECTION | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7.5 / Method: unknown / Details: Barford, D., (1994) Science, 263, 1397. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Wavelength: 1.541 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 15, 2002 / Details: YALE MIRRORS |
Radiation | Monochromator: YALE MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.541 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→25 Å / Num. obs: 26299 / % possible obs: 98.1 % / Redundancy: 6.98 % / Rmerge(I) obs: 0.071 / Net I/σ(I): 25.68 |
Reflection shell | Resolution: 2.15→2.2 Å / Rmerge(I) obs: 0.332 / Mean I/σ(I) obs: 3.97 / % possible all: 96.7 |
Reflection | *PLUS Highest resolution: 2.15 Å / Lowest resolution: 25 Å / Num. measured all: 183671 / Rmerge(I) obs: 0.071 |
Reflection shell | *PLUS % possible obs: 96.7 % / Rmerge(I) obs: 0.332 / Mean I/σ(I) obs: 3.97 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2HNP Resolution: 2.15→76.7 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.934 / SU B: 3.676 / SU ML: 0.098 / Cross valid method: THROUGHOUT / ESU R: 0.163 / ESU R Free: 0.151 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.6 Å2
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Refinement step | Cycle: LAST / Resolution: 2.15→76.7 Å
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Refine LS restraints |
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