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Open data
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Basic information
Entry | Database: PDB / ID: 1bey | ||||||
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Title | ANTIBODY TO CAMPATH-1H HUMANIZED FAB | ||||||
![]() | (CAMPATH-1H ANTIBODY) x 2 | ||||||
![]() | ANTIBODY / FAB / CAMPATH-1H / CD52 | ||||||
Function / homology | ![]() immunoglobulin complex, circulating / immunoglobulin receptor binding / complement activation, classical pathway / antigen binding / antibacterial humoral response / blood microparticle / immune response / extracellular space / extracellular exosome / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cheetham, G.M.T. / Hale, G. / Waldmann, H. / Bloomer, A.C. | ||||||
![]() | ![]() Title: Crystal structures of a rat anti-CD52 (CAMPATH-1) therapeutic antibody Fab fragment and its humanized counterpart. Authors: Cheetham, G.M. / Hale, G. / Waldmann, H. / Bloomer, A.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.6 KB | Display | ![]() |
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PDB format | ![]() | 66.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 373.4 KB | Display | ![]() |
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Full document | ![]() | 418.7 KB | Display | |
Data in XML | ![]() | 16 KB | Display | |
Data in CIF | ![]() | 23.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1bfoC ![]() 1fdlS ![]() 7fabS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Antibody | Mass: 23596.271 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: HYBRIDOMA CULTURE SUPERNATANT / Source: (natural) ![]() |
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#2: Antibody | Mass: 23418.307 Da / Num. of mol.: 1 / Fragment: FAB FRAGMENT / Source method: isolated from a natural source / Details: HYBRIDOMA CULTURE SUPERNATANT / Source: (natural) ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.4 Å3/Da / Density % sol: 58 % | ||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.0 | ||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 1, 1992 / Details: MIRRORS |
Radiation | Monochromator: MONOCHROMATOR / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 3.25→20 Å / Num. obs: 10139 / % possible obs: 92 % / Observed criterion σ(I): 1 / Redundancy: 3 % / Biso Wilson estimate: 39 Å2 / Rmerge(I) obs: 0.11 / Rsym value: 0.11 / Net I/σ(I): 8 |
Reflection shell | Resolution: 3.25→3.42 Å / Redundancy: 1.6 % / Rmerge(I) obs: 0.204 / Mean I/σ(I) obs: 3.8 / Rsym value: 0.204 / % possible all: 67 |
Reflection shell | *PLUS % possible obs: 86.6 % / Rmerge(I) obs: 0.227 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRIES 7FAB (CON) 1FDL (VAR) Resolution: 3.25→6 Å / Rfactor Rfree error: 0.014 / Isotropic thermal model: GROUPED, UNRESTRAINED / Cross valid method: THROUGHOUT / σ(F): 3
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Displacement parameters | Biso mean: 54.8 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.25→6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.25→3.35 Å / Rfactor Rfree error: 0.059 / Total num. of bins used: 10
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Xplor file | Serial no: 1 / Param file: CNS_TOPPAR:PROTEIN_REP.PA / Topol file: CNS_TOPPAR:PROTEIN.TOP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: 'CNS' / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.229 / Rfactor Rfree: 0.309 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.321 |