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- PDB-7fab: CRYSTAL STRUCTURE OF HUMAN IMMUNOGLOBULIN FRAGMENT FAB NEW REFINE... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7fab | |||||||||
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Title | CRYSTAL STRUCTURE OF HUMAN IMMUNOGLOBULIN FRAGMENT FAB NEW REFINED AT 2.0 ANGSTROMS RESOLUTION | |||||||||
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![]() | IMMUNE SYSTEM / IMMUNOGLOBULIN | |||||||||
Function / homology | ![]() IgD immunoglobulin complex / IgM immunoglobulin complex / IgA immunoglobulin complex / IgE immunoglobulin complex / CD22 mediated BCR regulation / immunoglobulin complex / IgG immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement ...IgD immunoglobulin complex / IgM immunoglobulin complex / IgA immunoglobulin complex / IgE immunoglobulin complex / CD22 mediated BCR regulation / immunoglobulin complex / IgG immunoglobulin complex / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / Initial triggering of complement / FCGR activation / immunoglobulin mediated immune response / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Scavenging of heme from plasma / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCERI mediated MAPK activation / FCGR3A-mediated phagocytosis / antigen binding / B cell receptor signaling pathway / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / adaptive immune response / Potential therapeutics for SARS / blood microparticle / immune response / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Saul, F. / Poljak, R.J. | |||||||||
![]() | ![]() Title: Crystal structure of human immunoglobulin fragment Fab New refined at 2.0 A resolution. Authors: Saul, F.A. / Poljak, R.J. #1: ![]() Title: Preliminary Refinement and Structural Analysis of the Fab Fragment from Human Immunoglobulin New at 2.0 Angstroms Resolution Authors: Saul, F.A. / Amzel, L.M. / Poljak, R.J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 94.7 KB | Display | ![]() |
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PDB format | ![]() | 71 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 369.8 KB | Display | ![]() |
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Full document | ![]() | 378.5 KB | Display | |
Data in XML | ![]() | 10 KB | Display | |
Data in CIF | ![]() | 16.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO L 137 / 2: CIS PROLINE - PRO H 151 / 3: CIS PROLINE - PRO H 153 | ||||||||
Details | THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT OF THIS STRUCTURE CONTAINS TWO HOMOLOGOUS, BUT DIFFERENT, POLYPEPTIDE CHAINS WHICH ARE DESIGNATED LIGHT (L) AND HEAVY (H). COORDINATES OF WATER MOLECULES (W) ARE INCLUDED. THESE COORDINATES REPLACE A PREVIOUS ENTRY. THE STRUCTURE WAS REFINED TO 2.0 ANGSTROMS RESOLUTION WITH A MORE COMPLETE DATA SET OBTAINED BY THE USE OF AN X-RAY AREA DETECTOR. |
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Components
#1: Antibody | Mass: 22073.605 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
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#2: Antibody | Mass: 22986.840 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.58 % | ||||||||||||||||||||
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Crystal grow | *PLUS Method: microdialysisDetails: referred to 'Rossi, G.', (1969) Nature., 223, 837-838 | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 6 Å / Num. obs: 28301 / Observed criterion σ(F): 2.5 / Num. measured all: 80611 |
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Processing
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Refinement | Rfactor Rwork: 0.169 / Rfactor obs: 0.169 / Highest resolution: 2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2 Å / Lowest resolution: 6 Å / Num. reflection obs: 28301 / σ(F): 2.5 / Rfactor obs: 0.169 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 3.03 |